Bacillus Cereus in Eggshell: Enterotoxigenic Profiles and Biofilm Production

A study was carried out with the objective of determining presence Bacillus cereus in eggshells commercialized Mexico, enterotoxigenic profile isolated strains, and production biofilms different materials as well eggshell. 1000 chicken eggs from four commercial brands were collected markets supermarkets located city Chilpancingo, Mexico. The molecular identification by amplification gyrB gene profiles cytK, ces, nheABC, hblABD genes, addition to tasA sipW genes associated biofilms. In eggshells, evaluated. microbiological analysis B. yielded a frequency 5.5% (55/1000), this higher brand III (11.6%, p=0.0001) white eggshell (7.6%, 38/500, p≤0.001) marketing source, it similar between market (5.2% / 26/500) supermarket (5.8%, 29/500). most common toxigenic (23/55). Biofilm is high PVC relation other (p<0.0001), related 72.7% 40% respectively; highest gene; eggshell, strains (54/55) able produce biofilm. Strains potential circulate persist product, which shows need for sanitary regulation country.